بررسی اطلاع‌دهندگی مارکر D9S1876 واقع در ناحیه ژنTMC1 در جمعیت ایرانی

Background and Objective: TMC1 gene mutations are known as the most common causes of autosomal recessive non-syndromic hearing loss (ARNSHL) in different populations. According to large size of the TMC1 gene and the large number of identified mutations in this gene, application of polymorphic markers is suggested for carrier detection and prenatal diagnosis in families. In this study, informativeness of D9S1876 STR marker with CA repeat was evaluated in five various ethnic groups of the Iranian population including Fars, Azeri, Turkmen, Gilak and Arab. Materials and Methods: The D9S1876 locus located within the TMC1 gene region was genotyped by polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and fluorescent capillary electrophoresis. The genotyping data from 165 unrelated healthy individuals were analyzed by GeneMarker HID Human STR Identity software, GenePop program and Microsatellite Tools software. 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